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KMID : 0613820070170020230
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Journal of Life Science
2007 Volume.17 No. 2 p.230 ~ p.234
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Expression of Cu/Zn SOD according to H₂O₂ in Hepatoma cell line
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Kim Young-Min
Seo Won-Sook
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Abstract
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Oxygen is required for many important aerobic cellular reactions, it may undergo electrontransfer reactions, which generate highly reactive membrane-toxic intermediates (reactive oxygen species, ROS), such as hydrogen peroxide, singlet oxygen, superoxide radical, hydroxyl radical, hydroperoxyl radical, hydroxy ion. Various mechanisms are available to protect cells against damage caused by oxidative free radicals, including scavenging enzyme systems such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx). This antioxidant defense system is a very complex and finely tuned system consisting of enzymes capable of detoxifying oxygen radicals as well as low molecular weight antioxidants. In addition, repair and turnover processes help to minimize subcellular damage resulting from free radical attack. H₂O₂, one of the major ROS, is produced at a high rate as a product of normal aerobic metabolism. The primary cellular enzymatic defense systems against H₂O₂ are the glutathione redox cycle and catalase. From Northern blot analysis of total RNAs from cultured cell with H₂O₂ treatment, various results were obtained. Expression of Cu/Zn SOD decreased when cell passage increased, but the level of the Cu/Zn SOD was scarcely expressed in 35 passage.
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KEYWORD
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ROS, Cu/Zn SOD, antioxidants, H₂O₂, hepatoma
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